6ED1
Bacteroides dorei Beta-glucuronidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-D |
| Synchrotron site | APS |
| Beamline | 23-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 242.663, 101.355, 168.600 |
| Unit cell angles | 90.00, 94.97, 90.00 |
Refinement procedure
| Resolution | 29.536 - 2.900 |
| R-factor | 0.1804 |
| Rwork | 0.178 |
| R-free | 0.26840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5uj6 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.059 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.540 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.118 | 0.417 |
| Number of reflections | 90006 | |
| <I/σ(I)> | 9.8 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 0.35 M sodium citrate, 16% PEG3350 |
