6ECO
Hexamer-2-Foldon HIV-1 capsid platform
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-25 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9798 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 95.250, 95.250, 122.570 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 4.200 |
| R-factor | 0.28766 |
| Rwork | 0.286 |
| R-free | 0.31638 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4xfx |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.349 |
| Data reduction software | XDS (20180126) |
| Data scaling software | XDS (20180126) |
| Phasing software | PHASER (7.0.0) |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 4.310 |
| High resolution limit [Å] | 4.200 | 4.200 |
| Rmerge | 0.110 | 0.900 |
| Number of reflections | 8601 | |
| <I/σ(I)> | 4.4 | 1.4 |
| Completeness [%] | 94.7 | |
| Redundancy | 3 | |
| CC(1/2) | 0.997 | 0.815 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICROBATCH | 7.5 | 293 | 0.2M Sodium Chloride, 0.1M HEPES pH 7.5, 12% PEG 8000 |
