6ECA

Lactobacillus rhamnosus Beta-glucuronidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 61 2 2
Unit cell lengths	154.800, 154.800, 241.906
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	29.497 - 2.853
R-factor	0.1522
Rwork	0.149
R-free	0.21250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lpg
RMSD bond length	0.008
RMSD bond angle	0.921
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX
Refinement software	PHENIX ((1.12_2829))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.500	2.970
High resolution limit [Å]	2.850	2.850
Number of reflections	374145
<I/σ(I)>	12.6
Completeness [%]	99.2
Redundancy	9.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293.15	17% PEG3350, 0.4 M NaCl, and and 0.1 Tris HCl pH 7.4