6EBP
Crystal Structure of the Class Ie Ribonucleotide Reductase Beta Subunit from Aerococcus urinae in Activated Form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2018-06-15 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 80.004, 109.417, 83.861 |
| Unit cell angles | 90.00, 90.06, 90.00 |
Refinement procedure
| Resolution | 35.180 - 1.590 |
| R-factor | 0.1637 |
| Rwork | 0.163 |
| R-free | 0.17930 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6ebo |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.059 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.620 |
| High resolution limit [Å] | 1.590 | 4.320 | 1.590 |
| Rmerge | 0.080 | 0.030 | 0.895 |
| Rmeas | 0.086 | 0.032 | 0.966 |
| Rpim | 0.031 | 0.011 | 0.360 |
| Total number of observations | 1407352 | ||
| Number of reflections | 193462 | 9866 | 9522 |
| <I/σ(I)> | 8.5 | ||
| Completeness [%] | 99.6 | 99.8 | 98.8 |
| Redundancy | 7.3 | 7.6 | 6.7 |
| CC(1/2) | 0.999 | 0.767 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 298 | 15% (w/v) PEG 4000, 0.3 M calcium chloride, 0.1 M Tris pH 8.5 |
