6EAC
Pseudomonas syringae SelO
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-02-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97914 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 136.174, 158.056, 227.248 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.136 - 2.269 |
| R-factor | 0.1822 |
| Rwork | 0.182 |
| R-free | 0.21170 |
| Structure solution method | SAD |
| RMSD bond length | 0.000 |
| RMSD bond angle | 0.490 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.140 | 2.310 |
| High resolution limit [Å] | 2.269 | 2.269 |
| Rmerge | 0.177 | 0.774 |
| Rpim | 0.063 | 0.292 |
| Number of reflections | 112381 | 5208 |
| <I/σ(I)> | 11.5 | 2.1 |
| Completeness [%] | 99.5 | 93.6 |
| Redundancy | 9.1 | 7.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.017 M Tris-HCl, 0.2 M calcium acetate, 0.1 M sodium chloride, 1 mM TCEP, 1 mM AMP-PNP, 22% PEG 3350, 30% ethylene glycol |
