6E9U
The crystal structure of bovine ultralong antibody BOV-7
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-02-23 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.978560 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.261, 79.245, 62.164 |
Unit cell angles | 90.00, 95.19, 90.00 |
Refinement procedure
Resolution | 40.770 - 2.295 |
R-factor | 0.2159 |
Rwork | 0.214 |
R-free | 0.25650 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.002 |
RMSD bond angle | 0.554 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.14_3211: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.790 | 2.420 |
High resolution limit [Å] | 2.290 | 2.290 |
Rmerge | 0.049 | 0.330 |
Number of reflections | 21713 | |
<I/σ(I)> | 15.4 | |
Completeness [%] | 99.5 | |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 291.15 | 7% PEG 8000, 14% isopropanol, 0.1 M Tris-HCl pH 7.5 |