6E9O
E. coli D-galactonate:proton symporter mutant E133Q in the outward substrate-bound form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-18 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 1.1158 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 217.070, 70.710, 107.160 |
| Unit cell angles | 90.00, 101.81, 90.00 |
Refinement procedure
| Resolution | 14.984 - 3.501 |
| R-factor | 0.2862 |
| Rwork | 0.285 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e9n |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.610 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 106.230 | 3.770 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmeas | 0.080 | 5.150 |
| Number of reflections | 20394 | 4030 |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 99.8 | |
| Redundancy | 3.5 | |
| CC(1/2) | 1.000 | 0.159 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 277 | 100mM glycine pH 9, 32% PEG 1000 |
