6E9C

Selenomethionine Derivative Structure of A Bacterial Homolog to Human Lysosomal Transporter, Spinster

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	93
Detector technology	PIXEL
Collection date	2017-06-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97775
Spacegroup name	P 21 21 21
Unit cell lengths	87.590, 87.330, 122.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.978 - 3.200
R-factor	0.2772
Rwork	0.277
R-free	0.28430
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.163
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.280
High resolution limit [Å]	3.200	3.200
Number of reflections	29749
<I/σ(I)>	10.27
Completeness [%]	99.6	99.7
Redundancy	9.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	277	32.5(v/v)% PEG 400, 100 mM Tris-HCl