6E8Z
Binary complex of Human glycerol 3-phosphate dehydrogenase with NAD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-03-05 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.97946 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 63.580, 78.780, 69.179 |
Unit cell angles | 90.00, 104.32, 90.00 |
Refinement procedure
Resolution | 33.960 - 2.100 |
R-factor | 0.1717 |
Rwork | 0.169 |
R-free | 0.22470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1x0x |
RMSD bond length | 0.012 |
RMSD bond angle | 1.160 |
Data reduction software | MOSFLM (7.2.1) |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX ((1.12_2829)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 33.960 | 2.175 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.047 | 0.195 |
Rmeas | 0.056 | 0.234 |
Number of reflections | 37384 | 3740 |
<I/σ(I)> | 13.5 | |
Completeness [%] | 96.6 | 98.1 |
Redundancy | 3.2 | 3.2 |
CC(1/2) | 0.998 | 0.966 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 287 | 200 mM potassium chloride, 23% PEG 20000, 0.1 M bis-tris propane pH 7.0, 10 mM NAD; 10 mg/mL |