6E8N
Crystal structure of glycosylated human EPDR1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-07-01 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.6300 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 102.107, 136.399, 75.908 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.363 - 3.200 |
| R-factor | 0.2266 |
| Rwork | 0.220 |
| R-free | 0.28260 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e7o |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.355 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3211)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.400 | 3.170 |
| High resolution limit [Å] | 3.110 | 3.110 |
| Number of reflections | 9779 | |
| <I/σ(I)> | 24 | 3.4 |
| Completeness [%] | 99.3 | 84.3 |
| Redundancy | 7 | 6.2 |
| CC(1/2) | 0.967 | 0.818 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG3350, 0.2 M zinc acetate |
