6E8M

Legionella Longbeachae LeSH (Llo2327) bound to the human DnaJ-A1 pTyr381 peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	114
Detector technology	IMAGE PLATE
Collection date	2013-05-23
Detector	MAR scanner 345 mm plate
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	40.900, 64.710, 67.850
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.210 - 1.610
R-factor	0.1992
Rwork	0.199
R-free	0.21230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e8h
RMSD bond length	0.006
RMSD bond angle	0.843
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	33.920	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.052	0.503
Number of reflections	22071	3192
<I/σ(I)>	24.9	4.1
Completeness [%]	90.3	91.2
Redundancy	11	10.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		296	PEG3000, malic acid, Tris-HCl