6E8D
Crystal structure of the Bacillus subtilis sliding clamp-MutL complex.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08B1-1 |
| Synchrotron site | CLSI |
| Beamline | 08B1-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-05-27 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.97951 |
| Spacegroup name | P 1 |
| Unit cell lengths | 59.020, 83.890, 128.260 |
| Unit cell angles | 80.30, 83.57, 89.96 |
Refinement procedure
| Resolution | 48.255 - 2.340 |
| R-factor | 0.2164 |
| Rwork | 0.216 |
| R-free | 0.25020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.732 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.255 | 2.420 |
| High resolution limit [Å] | 2.340 | 2.340 |
| Number of reflections | 98638 | |
| <I/σ(I)> | 13.4 | |
| Completeness [%] | 97.1 | |
| Redundancy | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM HEPES pH 7.5, 25 % PEG 3,350, 200 mM ammonium sulfate. |
