6E7D
Structure of the inhibitory NKR-P1B receptor bound to the host-encoded ligand, Clr-b
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-12-12 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9436 |
Spacegroup name | P 1 |
Unit cell lengths | 66.930, 122.069, 131.561 |
Unit cell angles | 73.12, 82.08, 84.46 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 66.100 | 3.060 |
High resolution limit [Å] | 2.900 | 2.900 |
Rmerge | 0.176 | 0.545 |
Rpim | 0.114 | 0.363 |
Number of reflections | 80841 | 12101 |
<I/σ(I)> | 6.3 | 2.2 |
Completeness [%] | 92.8 | 95.4 |
Redundancy | 2.9 | 2.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 21 % (w/v) PEG 3350 0.1 M Bis/Tris pH 5.9 5 % glycerol (v/v) 0.2 M ammonium sulfate |