6E71

Structure of Human Transthyretin Val30Met/Thr119Met Mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	P 21 21 2
Unit cell lengths	43.140, 86.540, 63.880
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.270 - 1.500
R-factor	0.193
Rwork	0.191
R-free	0.21200
RMSD bond length	0.010
RMSD bond angle	1.050
Data reduction software	XDS
Data scaling software	XSCALE
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.270	43.270	1.540
High resolution limit [Å]	1.500	6.710	1.500
Rmerge	0.047	0.020	0.907
Rmeas	0.056	0.025	1.083
Number of reflections	38230	488	2820
<I/σ(I)>	13.95	39.98	1.54
Completeness [%]	98.0	94.4	98.7
Redundancy	3.249	2.906	3.214
CC(1/2)	0.999	0.999	0.511

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	298	0.2M Lithium Sulfate, 0.1M Tris, 30% (w/v) PEG 4000