6E6P

HRAS G13D bound to GppNHp (Ha,b,c13GNP)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2014-04-14
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54178
Spacegroup name	P 21 21 21
Unit cell lengths	61.649, 75.567, 94.722
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.100 - 1.930
Rwork	0.184
R-free	0.23700
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.960
High resolution limit [Å]	1.930	5.240	1.930
Rmerge	0.085	0.046	0.622
Rmeas	0.092	0.050	0.686
Rpim	0.035	0.019	0.284
Total number of observations	217382
Number of reflections	32621	1852	1356
<I/σ(I)>	8.3
Completeness [%]	96.0	99.6	81.8
Redundancy	6.7	6.6	5
CC(1/2)		0.998	0.831

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	291.15	152 mM Calcium Acetate, 22.8% PEG 3350, 9.5% stabilization buffer (20 mM HEPES pH 7.5, 50 mM NaCl, 20 mM MgCl2), Crystals were grown in 2uL by 2 uL drops of mother liquor to protein (22 mg/mL), Cryoprotectant: 70% glycerol and 30% mother liquor