6E6M
Crystal structure of human cellular retinol-binding protein 1 in complex with cannabidiorcin (CBDO)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 80 |
Detector technology | CCD |
Collection date | 2018-03-13 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.210, 93.040, 119.820 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 73.487 - 1.550 |
R-factor | 0.1676 |
Rwork | 0.166 |
R-free | 0.20290 |
RMSD bond length | 0.009 |
RMSD bond angle | 1.002 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 73.490 | 1.580 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.089 | 0.800 |
Rmeas | 0.105 | 0.940 |
Rpim | 0.480 | |
Number of reflections | 61400 | 2931 |
<I/σ(I)> | 9 | 2.6 |
Completeness [%] | 100.0 | |
Redundancy | 6.5 | |
CC(1/2) | 0.997 | 0.850 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 295 | 0.1 m BisTris, pH 5.5, 25% PEG 3350 |