6E6H
NRAS G13D bound to GppNHp (N13GNP)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 173.15 |
Detector technology | IMAGE PLATE |
Collection date | 2014-07-08 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 59.553, 39.565, 65.016 |
Unit cell angles | 90.00, 106.40, 90.00 |
Refinement procedure
Resolution | 32.500 - 1.990 |
Rwork | 0.179 |
R-free | 0.23600 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
Rmerge | 0.098 | 0.058 | 0.567 |
Rmeas | 0.112 | 0.066 | 0.668 |
Rpim | 0.051 | 0.029 | 0.343 |
Total number of observations | 42385 | ||
Number of reflections | 9752 | 524 | 411 |
<I/σ(I)> | 8 | ||
Completeness [%] | 97.0 | 98.9 | 86.3 |
Redundancy | 4.3 | 4.6 | 3.4 |
CC(1/2) | 0.996 | 0.545 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291.15 | 9% 2-propanol, 72 mM HEPES, 20% PEG 4000, 15% stabilization buffer (20 mM HEPES, 50 mM NaCl, 20 mM MgCl2, at pH 7.5), Crystals were grown in 1uL by 1uL drops of mother liquor to protein (12.2 mg/mL) No cryoprotectant used for diffraction |