6E6B

Crystal structure of the Protocadherin GammaB4 extracellular domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-01
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97919
Spacegroup name	P 1 21 1
Unit cell lengths	127.730, 87.580, 149.330
Unit cell angles	90.00, 109.94, 90.00

Refinement procedure

Resolution	38.290 - 4.520
R-factor	0.2336
Rwork	0.231
R-free	0.27670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5v5x 5t9t
RMSD bond length	0.002
RMSD bond angle	0.565
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	5.050
High resolution limit [Å]	4.500	4.500
Rmerge	0.113	0.173
Rmeas	0.138	0.211
Rpim	0.078	0.119
Number of reflections	8694	317
<I/σ(I)>	5.2	5.7
Completeness [%]	93.4
Redundancy	2.8	3
CC(1/2)	0.995	0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.5	295	10% (w/v) PEG8000, 20% ethylene glycol, 10% Morpheus Amino Acids (Molecular Dimensions), and 0.1 M Morpheus Buffer System 2 (Hepes/MOPS buffer; Molecular Dimensions) pH 7.5