6E6A
Triclinic crystal form of IncA G144A point mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-09-21 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 |
| Unit cell lengths | 41.022, 43.533, 45.783 |
| Unit cell angles | 92.93, 95.96, 93.91 |
Refinement procedure
| Resolution | 14.960 - 1.950 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.21300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6e7e |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 2.020 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Number of reflections | 20042 | 1478 |
| <I/σ(I)> | 20.4 | 5.8 |
| Completeness [%] | 88.1 | 66.3 |
| Redundancy | 1.5 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.2 M sodium acetate trihydrate, pH 8.0, 20% w/v PEG3350 |
