6E65
Crystal structure of malaria transmission-blocking antibody TB31F
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-08-26 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.999977 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 55.680, 70.720, 115.360 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.698 - 1.500 |
R-factor | 0.1752 |
Rwork | 0.175 |
R-free | 0.18700 |
RMSD bond length | 0.006 |
RMSD bond angle | 0.899 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.700 | 1.600 |
High resolution limit [Å] | 1.500 | 1.500 |
Number of reflections | 73693 | 12770 |
<I/σ(I)> | 15.9 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13 | 13.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES, pH 7.5, 10% (v/v) 2-propanol, 20% (w/v) PEG 4000 |