6E60

Bacteroides ovatus mixed-linkage glucan utilization locus (MLGUL) SGBP-A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-11-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	47.279, 89.367, 120.941
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.250 - 1.500
R-factor	0.1591
Rwork	0.158
R-free	0.18400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	unpublished
RMSD bond length	0.012
RMSD bond angle	1.582
Data reduction software	XDS
Phasing software	PHASER
Refinement software	REFMAC (5.8.0230)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.590
High resolution limit [Å]	1.500	1.500
Number of reflections	81232	12744
<I/σ(I)>	9.84	1.49
Completeness [%]	97.6	95.9
Redundancy	4.48	4.49
CC(1/2)	0.997	0.605

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	296	0.2M magnesium chloride, 0.1M bis-tris, 25% (w/v) PEG3350