6E5U
Crystal structure of the mRNA export receptor NXF1/NXT1 in complex with influenza virus NS1 protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-F |
| Synchrotron site | APS |
| Beamline | 21-ID-F |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-15 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 65 |
| Unit cell lengths | 101.905, 101.905, 949.476 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 44.126 - 3.800 |
| R-factor | 0.2375 |
| Rwork | 0.236 |
| R-free | 0.28250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4wyk 3rw6 4wyk 4opa |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.880 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.14_3260: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.870 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.139 | 1.423 |
| Number of reflections | 54178 | 2660 |
| <I/σ(I)> | 20.1 | 1 |
| Completeness [%] | 98.3 | 98 |
| Redundancy | 7.7 | 6.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 293 | 50 mM Sodium Citrate (pH 5.6), 6.5% PEG3350, 0.1 M NaCl, 0.1 M KCl |
