6E5F

Crystal structure of LpqN involved in cell envelope biogenesis of Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-06-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9792
Spacegroup name	I 4
Unit cell lengths	80.004, 80.004, 59.239
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.570 - 1.370
R-factor	0.178
Rwork	0.177
R-free	0.20350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6e5d
RMSD bond length	0.004
RMSD bond angle	0.764
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14rc1_3177)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.571	1.380
High resolution limit [Å]	1.350	1.350
Rmerge	0.058	1.264
Rmeas	0.062	1.510
Rpim	0.021	0.806
Number of reflections	40174
<I/σ(I)>	18.2
Completeness [%]	98.4	70.6
Redundancy	8.4	3.2
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	0.1 M sodium citrate, pH 6.5, 1.9 M ammonium sulfate