6E52
Chimeric structure of Saccharomyces cerevisiae GCN4 leucine zipper fused to Staphylococcus aureus AgrC cytoplasmic histidine kinase module (dataset anisotropically truncated by STARANISO)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-11-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 63.141, 83.375, 146.966 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.930 |
| R-factor | 0.22081 |
| Rwork | 0.218 |
| R-free | 0.26807 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.521 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 31.760 | 1.980 |
| High resolution limit [Å] | 1.930 | 1.930 |
| Rmeas | 0.087 | 1.405 |
| Rpim | 0.035 | 0.655 |
| Number of reflections | 36936 | 312 |
| <I/σ(I)> | 11.9 | 1.2 |
| Completeness [%] | 61.6 | 7.3 |
| Redundancy | 6.4 | 4.4 |
| CC(1/2) | 0.990 | 0.490 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 295 | 0.1 M Tris pH 8.0 and 2.8 M NaCl |
