6E4R
Crystal Structure of the Drosophila Melanogaster Polypeptide N-Acetylgalactosaminyl Transferase PGANT9B
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-BM |
| Synchrotron site | APS |
| Beamline | 22-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-11-04 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 113.913, 48.440, 232.037 |
| Unit cell angles | 90.00, 91.40, 90.00 |
Refinement procedure
| Resolution | 20.070 - 2.061 |
| R-factor | 0.1665 |
| Rwork | 0.164 |
| R-free | 0.20680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1xhb |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.973 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 20.070 | 20.070 | 2.100 |
| High resolution limit [Å] | 2.060 | 10.310 | 2.060 |
| Total number of observations | 76401 | ||
| Number of reflections | 76401 | 549 | 3479 |
| <I/σ(I)> | 6.7 | ||
| Completeness [%] | 96.9 | 79.7 | 73.7 |
| Redundancy | 1 | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 20% PEG3350 and 0.18 M ammonium citrate |
