6E4Q
Crystal Structure of the Drosophila Melanogaster Polypeptide N-Acetylgalactosaminyl Transferase PGANT9A in Complex with UDP and Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-10-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0 |
| Spacegroup name | I 1 2 1 |
| Unit cell lengths | 126.286, 168.756, 153.099 |
| Unit cell angles | 90.00, 106.28, 90.00 |
Refinement procedure
| Resolution | 19.954 - 2.799 |
| R-factor | 0.1931 |
| Rwork | 0.190 |
| R-free | 0.25960 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.160 |
| Data reduction software | HKL-2000 |
| Data scaling software | Aimless (0.5.15) |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 19.950 | 19.950 | 2.860 |
| High resolution limit [Å] | 2.800 | 13.420 | 2.800 |
| Total number of observations | 71031 | ||
| Number of reflections | 71031 | 500 | 3366 |
| <I/σ(I)> | 4.9 | ||
| Completeness [%] | 93.8 | 70.1 | 69.2 |
| Redundancy | 1 | 1 | 1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 292 | 22% PEG3350, 0.2 M sodium malonate,20 mM strontium chloride hexahydrate |
