6E2J

Crystal structure of the heterocomplex between human keratin 1 coil 1B containing S233L mutation and wild-type human keratin 10 coil 1B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-06
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9795
Spacegroup name	P 64 2 2
Unit cell lengths	93.296, 93.296, 124.742
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.648 - 2.386
R-factor	0.2723
Rwork	0.271
R-free	0.29430
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.004
RMSD bond angle	0.587
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.430
High resolution limit [Å]	2.386	6.480	2.390
Rmerge	0.139	0.097	0.969
Rmeas	0.145	0.102	1.019
Rpim	0.040	0.029	0.309
Number of reflections	13342	783	634
<I/σ(I)>	4.1
Completeness [%]	99.9	99.9	99.5
Redundancy	13.3	12.2	10
CC(1/2)		0.998	0.787

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.1 M Tris, 1.5 M ammonium sulfate, 12% glycerol