6E29

Crystal structure of Myceliophteria_thermophila Cps50 (Swd1) beta-propeller domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 17-ID
Synchrotron site	APS
Beamline	17-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2018-02-01
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.07812
Spacegroup name	P 1 21 1
Unit cell lengths	59.784, 88.900, 124.319
Unit cell angles	90.00, 94.30, 90.00

Refinement procedure

Resolution	45.026 - 1.818
R-factor	0.1701
Rwork	0.168
R-free	0.21560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2h13
RMSD bond length	0.014
RMSD bond angle	1.316
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.100	1.860
High resolution limit [Å]	1.818	1.818
Number of reflections	116058
<I/σ(I)>	13.8
Completeness [%]	92.0
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM sodium citrate, 22% (w/v) polyethylene glycol 3350