6E16

Ternary structure of c-Myc-TBP-TAF1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	80
Detector technology	CCD
Collection date	2018-03-20
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97934
Spacegroup name	P 43
Unit cell lengths	72.816, 72.816, 78.819
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.408 - 2.400
R-factor	0.1785
Rwork	0.177
R-free	0.21990
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	40BA
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.408	2.510
High resolution limit [Å]	2.400	2.400
Number of reflections	16091
<I/σ(I)>	13.75
Completeness [%]	99.4
Redundancy	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE		291	20% (w/w) PEG8000, 0.2M ammonium sulfare, and 0.1M HEPES pH7.5