6E0W
Crystal structure of the colanidase tailspike protein gp150 of Phage Phi92 complexed with one repeating unit of colanic acid
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2012-03-22 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9919 |
| Spacegroup name | H 3 |
| Unit cell lengths | 117.623, 117.623, 308.282 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 61.461 - 1.803 |
| R-factor | 0.1534 |
| Rwork | 0.153 |
| R-free | 0.17650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4RMX |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.172 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_3092: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.000 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.076 | 0.539 |
| Rmeas | 0.096 | 0.491 |
| Number of reflections | 281287 | 44765 |
| <I/σ(I)> | 7.47 | 1.71 |
| Completeness [%] | 95.8 | 94.3 |
| Redundancy | 2.6 | 2.4 |
| CC(1/2) | 0.991 | 0.848 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291 | 10% PEG 8000, 0.2M SrCl2, 0.1M MES pH 6.0, 10mg/ml |
