Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-09-13 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 127.120, 206.250, 211.840 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 50.010 - 2.600 |
| R-factor | 0.1885 |
| Rwork | 0.187 |
| R-free | 0.21132 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5VFL |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.734 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0216) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.010 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.153 | 1.445 |
| Number of reflections | 171050 | 8374 |
| <I/σ(I)> | 10.5 | 1.5 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7.3 | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2 M AMMONIUM CITRATE TRIBASIC 20% W/V PEG 3350 0.5 MM AG1, PH 7.0, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 293K |
