6DYS

C-terminal condensation domain of Ebony in complex with beta-alanyl-dopamine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX1
Synchrotron site	Australian Synchrotron
Beamline	MX1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-10-13
Detector	ADSC QUANTUM 210r
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	44.481, 56.494, 49.934
Unit cell angles	90.00, 113.50, 90.00

Refinement procedure

Resolution	45.790 - 2.300
R-factor	0.174
Rwork	0.172
R-free	0.20490
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dym
RMSD bond length	0.004
RMSD bond angle	0.647
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.13_2998)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.790	2.380
High resolution limit [Å]	2.300	2.300
Number of reflections	10138
<I/σ(I)>	14.2
Completeness [%]	99.4
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	293	HEPES, PEG400, CaCl2