6DX5

Crystal structure of the viral OTU domain protease from Farallon virus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-03-01
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 41 21 2
Unit cell lengths	70.276, 70.276, 176.258
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.941 - 2.224
R-factor	0.1651
Rwork	0.163
R-free	0.20250
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4hxd
RMSD bond length	0.015
RMSD bond angle	1.228
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.260
High resolution limit [Å]	2.220	2.220
Number of reflections	22465	2182
<I/σ(I)>	18.9	2.8
Completeness [%]	99.6
Redundancy	6.9
CC(1/2)	0.990	0.890

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.3 M magnesium chloride, 0.1 M MES pH 6.5, 8% PEG 4000