6DWW
Hermes transposase deletion dimer complex with (A/T) DNA and Mn2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2013-07-31 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 1.5498031 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 122.480, 136.460, 104.610 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.803 - 2.851 |
| R-factor | 0.202 |
| Rwork | 0.199 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4d1q |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.644 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | AMoRE |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.920 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.066 | 0.618 |
| Number of reflections | 20430 | 1476 |
| <I/σ(I)> | 20.44 | |
| Completeness [%] | 99.7 | |
| Redundancy | 7.31 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277 | 50 mM MES pH 5.8-6.5, 50 mM sodium acetate, 15% PEG MME 2000 |
