6DVI
Wild-type Lactate Monooxygenase from Mycobacterium smegmatis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1996-05-16 |
| Detector | PRINCETON 2K |
| Wavelength(s) | 0.908 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 148.400, 148.400, 272.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 77.494 - 2.300 |
| R-factor | 0.2233 |
| Rwork | 0.218 |
| R-free | 0.27200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.340 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 77.490 | 2.380 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 123105 | |
| <I/σ(I)> | 10.4 | 2.6 |
| Completeness [%] | 91.0 | 79.6 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | 0.1 M sodium citrate, pH 4.6, 0.4 M lithium sulfate, 0.4 M ammonium sulfate |
