6DVH
Lactate Monooxygenase from Mycobacterium smegmatis - C203A mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-04-15 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 149.620, 149.620, 274.250 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.903 - 1.700 |
| R-factor | 0.1716 |
| Rwork | 0.169 |
| R-free | 0.19440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.112 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.900 | 1.740 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 338277 | 24764 |
| <I/σ(I)> | 15.5 | 0.6 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 26.7 | 27.5 |
| CC(1/2) | 1.000 | 0.230 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 278 | 0.1 M sodium citrate, pH 4.6, 0.4 M lithium sulfate, 0.4 M ammonium sulfate |
