6DVB
Crystal structure of Mycobacterium tuberculosis transcription initiation complex(ECF sigma factor L) containing 5nt RNA with 5nt spacer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-2 |
| Synchrotron site | SSRL |
| Beamline | BL9-2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-01 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97946 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 143.658, 160.580, 240.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 52.247 - 3.800 |
| R-factor | 0.2035 |
| Rwork | 0.202 |
| R-free | 0.24070 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.457 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.21) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.800 | 3.910 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.211 | 0.942 |
| Number of reflections | 53728 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 97.1 | 96.5 |
| Redundancy | 8.2 | 8.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.7 | 295 | 100 mM sodium citrate tribasic dihydrate, pH 5.7, 200 mM sodium acetate, and 10% PEG4000 |
