6DV9
Crystal structure of Mycobacterium tuberculosis transcription initiation complex(ECF sigma factor L) containing 5nt RNA with 4nt spacer
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-12 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 143.270, 161.440, 237.690 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 91.471 - 3.800 |
| R-factor | 0.1868 |
| Rwork | 0.184 |
| R-free | 0.23200 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.489 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless (0.5.21) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 97.690 | 3.910 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmerge | 0.330 | 1.967 |
| Number of reflections | 53020 | |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 96.6 | 90.5 |
| Redundancy | 10.7 | 9.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 295 | 100 mM sodium citrate tribasic dihydrate, pH 5.5, 200 mM sodium acetate, and 10% PEG4000 |
