6DV5
Oligomeric complex of a Hsp27 24-mer at 3.6 A resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-04-21 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.0971 |
| Spacegroup name | H 3 |
| Unit cell lengths | 324.700, 324.700, 198.761 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 93.730 - 3.580 |
| R-factor | 0.23773 |
| Rwork | 0.238 |
| R-free | 0.24157 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ygd |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.596 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 93.730 | 3.672 |
| High resolution limit [Å] | 3.579 | 3.579 |
| Number of reflections | 87279 | |
| <I/σ(I)> | 2.7 | |
| Completeness [%] | 99.8 | |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294.15 | 0.5 M ammonium phosphate monobasic and 40 % tert-butanol. |
