6DV1

Crystal structure of the alpha-E-catenin actin-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-15
Detector	RAYONIX MX-300
Wavelength(s)	1.0, 0.9198
Spacegroup name	P 21 21 21
Unit cell lengths	56.292, 80.607, 104.488
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.150 - 2.200
R-factor	0.2106
Rwork	0.206
R-free	0.25230
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	0.842
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.150	2.280
High resolution limit [Å]	2.200	2.200
Rmerge	0.059	0.522
Number of reflections	24938
<I/σ(I)>	31.7	3.8
Completeness [%]	100.0	100
Redundancy	7.4	7.4
CC(1/2)	0.990	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.2 M potassium bromide, 2.2 M ammonium sulfate, 3% w/v D-galactose