6DU7
Glutathione reductase from Streptococcus pneumoniae
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-29 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.954 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.748, 172.671, 135.434 |
| Unit cell angles | 90.00, 91.05, 90.00 |
Refinement procedure
| Resolution | 46.600 - 2.560 |
| R-factor | 0.1826 |
| Rwork | 0.180 |
| R-free | 0.23250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ger |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.079 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0131) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.600 | 2.600 |
| High resolution limit [Å] | 2.560 | 2.560 |
| Number of reflections | 141364 | |
| <I/σ(I)> | 8.7 | |
| Completeness [%] | 98.9 | |
| Redundancy | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 0.1 M Bis-Tris propane, pH 7.0, 0.2 M potassium thiocyanate, 14% PEG3350, 0.1 mM NADP+ |
