6DU5

Crystal structure of hMettl16 catalytic domain in complex with MAT2A 3'UTR hairpin 6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-09-01
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.97934
Spacegroup name	P 41 21 2
Unit cell lengths	134.918, 134.918, 76.208
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	38.731 - 3.006
R-factor	0.2485
Rwork	0.248
R-free	0.26480
Structure solution method	SAD
RMSD bond length	0.004
RMSD bond angle	0.794
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX ((1.10_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.110
High resolution limit [Å]	3.000	3.000
Rmerge	0.074	1.226
Number of reflections	14533	1417
<I/σ(I)>	46	2
Completeness [%]	100.0	100
Redundancy	25	22

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1M Tris (pH 8.5), 20% glycerol, and 19% polyethylene (PEG) 10000