6DTV

Crystal structure of eukaryotic DNA primase large subunit iron-sulfur cluster domain Y395F mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2016-12-08
Detector	RAYONIX MX-300
Wavelength(s)	0.97872
Spacegroup name	P 21 21 21
Unit cell lengths	40.990, 50.873, 89.911
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.440 - 1.120
R-factor	0.125
Rwork	0.125
R-free	0.13700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6dtz
Data reduction software	DENZO
Data scaling software	HKL-2000
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.140
High resolution limit [Å]	1.100	1.100
Rmerge	0.054	0.465
Rmeas	0.060	0.041
Rpim	0.026	0.018
Total number of observations	360511
Number of reflections	72580	7757
<I/σ(I)>	14.6
Completeness [%]	93.7	43.6
Redundancy	5	3.9
CC(1/2)		0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	289	MPD, TRIS 8.5