6DTD
High-resolution crystal structure of Cas13b from Prevotella buccae
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 93.15 |
Detector technology | PIXEL |
Collection date | 2018-05-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.03320 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 90.821, 124.646, 140.728 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.654 - 1.650 |
R-factor | 0.1548 |
Rwork | 0.153 |
R-free | 0.18500 |
Structure solution method | MAD |
Data scaling software | XDS |
Phasing software | AutoSol |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.654 | 46.654 | 1.380 |
High resolution limit [Å] | 1.340 | 6.000 | 1.340 |
Rmerge | 0.127 | 0.071 | |
Rmeas | 0.132 | 0.074 | |
Total number of observations | 3076708 | ||
Number of reflections | 209453 | 4296 | 2464 |
<I/σ(I)> | 7.57 | 31.75 | |
Completeness [%] | 80.5 | 100 | 9.5 |
Redundancy | 10.782 | 12.941 | 1.354 |
CC(1/2) | 0.998 | 0.997 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 293.15 | salt, PEG |