6DT1

Crystal structure of the ligase from bacteriophage T4 complexed with DNA intermediate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2013-11-10
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97918
Spacegroup name	P 1
Unit cell lengths	63.526, 67.429, 114.635
Unit cell angles	88.78, 80.94, 62.97

Refinement procedure

Resolution	113.021 - 2.750
R-factor	0.1723
Rwork	0.170
R-free	0.21910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1x9n
RMSD bond length	0.006
RMSD bond angle	0.796
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.14rc1_3177: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	113.572	2.850
High resolution limit [Å]	2.750	2.750
Rmerge	0.078	0.540
Number of reflections	42792	4259
<I/σ(I)>	9.3	1.4
Completeness [%]	98.6	98.1
Redundancy	2	2
CC(1/2)	0.988	0.622

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	PEG3350