6DRX
Structural Determinants of Activation and Biased Agonism at the 5-HT2B Receptor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-08-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.033 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 59.380, 118.550, 168.203 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.257 - 3.100 |
| R-factor | 0.2453 |
| Rwork | 0.243 |
| R-free | 0.28590 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | : 4IB4 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.761 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.170 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Rmerge | 0.122 | 0.950 |
| Number of reflections | 10701 | 699 |
| <I/σ(I)> | 10.9 | 1.1 |
| Completeness [%] | 97.1 | 97.1 |
| Redundancy | 4.4 | 4.3 |
| CC(1/2) | 0.996 | 0.462 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 100 mM Tris/HCl pH 7.4-7.7, 30-50 mM Ammonium tartrate dibasic, 30% v/v PEG400 |
