6DRT

Crystal structure of the processivity clamp GP45 complexed with recognition peptide of ligase from bacteriophage T4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	CCD
Collection date	2014-11-26
Detector	ADSC QUANTUM 315
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	63.757, 90.950, 151.403
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.783 - 2.117
R-factor	0.2009
Rwork	0.199
R-free	0.24030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1czd
RMSD bond length	0.001
RMSD bond angle	0.368
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14rc1_3161)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	63.760	2.230
High resolution limit [Å]	2.117	2.117
Rmerge	0.061	1.340
Number of reflections	50401	4893
<I/σ(I)>	15.7	1.2
Completeness [%]	99.1	98.8
Redundancy	2.6	5.1
CC(1/2)	0.999	0.562

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	PEG3350