6DR4
X-ray crystallographic structure of a covalent trimer derived from A-beta 17_36 containing the I31V point mutation
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 123 |
| Detector technology | CCD |
| Collection date | 2018-05-02 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 63 2 2 |
| Unit cell lengths | 57.685, 57.685, 94.683 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 27.591 - 2.105 |
| R-factor | 0.2299 |
| Rwork | 0.226 |
| R-free | 0.26380 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.201 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.12_2829: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.340 | 2.170 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Number of reflections | 5880 | 461 |
| <I/σ(I)> | 35.7 | 2.7 |
| Completeness [%] | 99.9 | 99.1 |
| Redundancy | 17.6 | 13.9 |
| CC(1/2) | 1.000 | 0.886 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 296.15 | 0.1 M tirs pH 9.0, 0.2 M magnesium chloride, 2.7 M 1,6-hexanediol |
