6DQW

Flavobacterium johnsoniae class Id ribonucleotide reductase alpha subuint

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	100
Detector technology	CCD
Collection date	2015-10-12
Detector	RAYONIX MX-225
Wavelength(s)	0.97872
Spacegroup name	P 61
Unit cell lengths	161.031, 161.031, 187.733
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.530 - 2.600
R-factor	0.2084
Rwork	0.206
R-free	0.25080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2wgh
RMSD bond length	0.006
RMSD bond angle	1.037
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	BALBES
Refinement software	REFMAC (5.8.0222)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.600	7.050	2.600
Rmerge	0.100	0.026	0.905
Rmeas	0.104	0.027	0.946
Rpim	0.031	0.008	0.276
Number of reflections	85045	4336	4260
<I/σ(I)>	8
Completeness [%]	100.0	100	100
Redundancy	11.6	11.3	11.6
CC(1/2)		0.999	0.856

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.25 M magnesium chloride, 0.1 M Tris pH 7.0, 10% (w/v) PEG 8000