6DQP

Crystal structure of SsuE FMN reductase Delta118 mutant in apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-05
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	P 21 21 21
Unit cell lengths	40.918, 41.812, 182.256
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.807 - 1.546
R-factor	0.1612
Rwork	0.160
R-free	0.17840
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pty
RMSD bond length	0.009
RMSD bond angle	1.032
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX ((1.12_2829: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.807	1.580
High resolution limit [Å]	1.546	1.546
Number of reflections	46844
<I/σ(I)>	19.3
Completeness [%]	99.9
Redundancy	8.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM CHES: NaOH pH 9.5, 30 % (w/v) PEG 3000